Navigating the Tree of Life: Steps to Read a Phylogenetic Trees

Monika Mate
4 min readJul 6, 2023

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A phylogenetic tree is a graphical representation of the evolutionary relationships between different organisms. It is a powerful tool that can be used to infer evolutionary history, reconstruct the relationships between different taxa, and make predictions about the characteristics of different organisms.

What is a Phylogenetic Tree?

A phylogenetic tree is a branching diagram that shows the evolutionary relationships among species or groups of organisms. It is often depicted as a diagram with branches, nodes, and tips/terminal nodes. The branches represent the lineages or evolutionary paths of species, while the nodes indicate their common ancestors. The tips of the tree represent the present-day species or groups.

Phylogenetic trees can be seen in various types, each serving a distinct purpose and conveying unique information. These different tree types highlight specific features of the complex network of evolutionary relationships. For example — Rooted and unrooted trees primarily focus on capturing evolutionary connections, while phylograms, chronograms, cladograms, and meta-phylogenetic trees provide additional insights into clustering, evolutionary change, divergence times, and shared characteristics. By utilizing these diverse tree types, researchers can gain a broad understanding of the nature of evolution.

Types of Phylogenetic Trees

Why use Phylogenetic Trees?

Phylogenetic trees are essential in evolutionary biology and provide a visual representation of the relationships between species. They help to understand how species have evolved over time and how they are related to one another. By studying these trees, we can unravel the fascinating story of life’s evolution on our planet.

How to read a Phylogenetic tree?

Reading a phylogenetic tree can be a challenge, but with some fundamental steps and a little practice, it becomes easier. Here are a few fundamental points to consider when trying to read a phylogenetic tree:

Step 1: Understanding the Structure

The reading of phylogenetic trees starts by examining the overall structure of the tree which consists of branches, nodes and terminal nodes (tips/leaves). The branches represent evolutionary relationships, the nodes indicate common ancestors, and the terminal nodes represent observed species.

Understanding the Structure of Phylogenetic Tree

Step 2: Identifying the Root and Branches

The root of the tree is usually illustrated as the starting point. From the root, the branches extent outward, representing the evolutionary relationships among the species. Phylogenetic trees can be categorized as rooted (a designated root node represents a common ancestor of the organisms being studied) or unrooted trees (lack a designated root node).

Step 3: Evaluating the Branch Lengths

The length of the branches indicates the extent of evolutionary changes. Longer branches indicate more evolutionary changes or a longer time span since the common ancestor.

Step 4: Determining Relationships

To determine the relationships, examine the nodes where branches diverge or converge. Nodes represent common ancestors of the species connected by the branches. The closer the two species are, the more recently they shared a common ancestor. Clusters of species connected by a node are called clades. A clade is defined as a group of organisms that includes a single ancestor and all its descendent.

Determining Relations

Step 5: Analyzing tips and Taxonomy.

The terminal nodes/tips of the tree represent the observed species. These tips are labelled with their scientific names or assigned unique identifiers. The grouping of species is based on their evolutionary relatedness.

Analyzing tips and Taxonomy

Step 6: Branching Patterns

The branching patterns in the tree show a hierarchical pattern with larger clades branching into smaller ones. It is important to understand that the branch lengths on a phylogenetic tree do not indicate the time of divergence between different organisms. Instead, they indicate the amount of genetic difference between the organisms.

Conclusion:

By following these steps, various components of phylogenetic trees can be interpreted and will help to understand the relationships between different organisms and make predictions about the characteristics of different organisms based on their position on the tree.

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